Considerations regarding use of solvents in in vitro cell based assays
نویسندگان
چکیده
منابع مشابه
Effects of solvents and dosing procedure on chemical toxicity in cell-based in vitro assays.
Due to the implementation of new legislation, such as REACh, a dramatic increase of animal use for toxicity testing is expected and the search for alternatives is timely. Cell-based in vitro assays are promising alternatives. However, the behavior of chemicals in these assays is still poorly understood. We set out to quantify the exposure and associated toxicity of chemicals with different phys...
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A new antibiotic, aterrimin (Lewis et al., 1953), is unstable in aqueous solution at pH 7, and insoluble at lower pH levels. Aterrimin can be dissolved in absolute alcohol and the solutions can be used satisfactorily in a cylinder-plate type of assay (Heatley, 1944). Further investigation has established that sevbral other organic solvents are nontoxic to various bacteria when used in cylinders...
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in the present study, the effect of e-64 at different concentrations (0.5, 1, 5 and 10 µm) added to (1) the ivm medium on oocyte nuclear maturation and developmental competence of ovine oocytes, and (2) to the ivc medium on embryonic development of ovine embryos were investigated.
Practical considerations regarding the use of genotype
22 Genome-wide association studies of complex traits are often complicated by relatedness 23 among individuals. Ignoring or inappropriately accounting for relatedness often results in 24 inflated type I error rates. Either genotype or pedigree data can be used to estimate relat25 edness for use in mixed-models when undertaking QTL mapping. We performed simulations 26 to investigate methods for ...
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ژورنال
عنوان ژورنال: Cytotechnology
سال: 2013
ISSN: 0920-9069,1573-0778
DOI: 10.1007/s10616-012-9530-6